plasmids containing open reading frames (orfs) of human glut2 Search Results


human liver total rna  (TaKaRa)
95
TaKaRa human liver total rna
Human Liver Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human liver total rna/product/TaKaRa
Average 95 stars, based on 1 article reviews
human liver total rna - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
glut2 expression  (OriGene)
90
OriGene glut2 expression
(a) Schematic of the biochemical processes inside the AβCs. GOx, glucose oxidase; CAT, catalase; square brackets denote concentration. (b) Top, ISVs stained with uranyl acetate and imaged by TEM. The ISVs were used to mimic the insulin granules inside natural beta cells (scale bar, 100 nm). Bottom, size-distribution histogram of the insulin-containing ISVs. (c) Top, cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 5 μm). Bottom, size distribution of vesicles-in-vesicle superstructures (ISVs@OLV). (d) Magnified fractured cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 200 nm). From the fracture in d, small liposomal vesicles can be clearly seen inside the large liposome. (e) CLSM micrographs verifying the encapsulation of glucose oxidase labeled with fluorescein isothiocyanate (GOx-FITC) and catalase labeled with rhodamine B (CAT-RB) inside the large liposomes (scale bar, 5 μm). (f) Western blotting results indicating the retention of immunoreactivity of <t>GLUT2</t> in the superstructures. The full gel is shown in Supplementary Figure 9. (g) CLSM image showing the reconstitution of GLUT2 labeled with rhodamine B on the membranes of the larger liposomes (scale bar, 5 μm). (h) CLSM image showing insertion of the proton channel gramicidin A labeled with lysine-5-carboxyfluorescein into the outer membrane (scale bar, 5 μm).
Glut2 Expression, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glut2 expression/product/OriGene
Average 90 stars, based on 1 article reviews
glut2 expression - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier
cdna encoding mouse glut2  (OriGene)
99
OriGene cdna encoding mouse glut2
(a) Schematic of the biochemical processes inside the AβCs. GOx, glucose oxidase; CAT, catalase; square brackets denote concentration. (b) Top, ISVs stained with uranyl acetate and imaged by TEM. The ISVs were used to mimic the insulin granules inside natural beta cells (scale bar, 100 nm). Bottom, size-distribution histogram of the insulin-containing ISVs. (c) Top, cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 5 μm). Bottom, size distribution of vesicles-in-vesicle superstructures (ISVs@OLV). (d) Magnified fractured cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 200 nm). From the fracture in d, small liposomal vesicles can be clearly seen inside the large liposome. (e) CLSM micrographs verifying the encapsulation of glucose oxidase labeled with fluorescein isothiocyanate (GOx-FITC) and catalase labeled with rhodamine B (CAT-RB) inside the large liposomes (scale bar, 5 μm). (f) Western blotting results indicating the retention of immunoreactivity of <t>GLUT2</t> in the superstructures. The full gel is shown in Supplementary Figure 9. (g) CLSM image showing the reconstitution of GLUT2 labeled with rhodamine B on the membranes of the larger liposomes (scale bar, 5 μm). (h) CLSM image showing insertion of the proton channel gramicidin A labeled with lysine-5-carboxyfluorescein into the outer membrane (scale bar, 5 μm).
Cdna Encoding Mouse Glut2, supplied by OriGene, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cdna encoding mouse glut2/product/OriGene
Average 99 stars, based on 1 article reviews
cdna encoding mouse glut2 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
pbluescript ii ks(+) plasmids with open reading frames (orfs) of human glut2 (ohu20407 nm_000340.2)  (GenScript corporation)
90
GenScript corporation pbluescript ii ks(+) plasmids with open reading frames (orfs) of human glut2 (ohu20407 nm_000340.2)
Effect of BG on glucose uptake in oocytes expressing human <t>SGLT1.</t> Oocytes expressing human SGLT1 and oocytes injected with water (SHAM, negative control) were incubated in buffer containing radiolabeled glucose only (control) or buffers containing different amounts barley BG (BG-HMW: 650,000 average molecular weight; BG-LMW: 229,000 average molecular weight). Data are means ± SEM ( n = 10–12 oocytes per treatment). *Significantly different from all other treatment groups p < 0.0001.
Pbluescript Ii Ks(+) Plasmids With Open Reading Frames (Orfs) Of Human Glut2 (Ohu20407 Nm 000340.2), supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbluescript ii ks(+) plasmids with open reading frames (orfs) of human glut2 (ohu20407 nm_000340.2)/product/GenScript corporation
Average 90 stars, based on 1 article reviews
pbluescript ii ks(+) plasmids with open reading frames (orfs) of human glut2 (ohu20407 nm_000340.2) - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

Image Search Results


(a) Schematic of the biochemical processes inside the AβCs. GOx, glucose oxidase; CAT, catalase; square brackets denote concentration. (b) Top, ISVs stained with uranyl acetate and imaged by TEM. The ISVs were used to mimic the insulin granules inside natural beta cells (scale bar, 100 nm). Bottom, size-distribution histogram of the insulin-containing ISVs. (c) Top, cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 5 μm). Bottom, size distribution of vesicles-in-vesicle superstructures (ISVs@OLV). (d) Magnified fractured cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 200 nm). From the fracture in d, small liposomal vesicles can be clearly seen inside the large liposome. (e) CLSM micrographs verifying the encapsulation of glucose oxidase labeled with fluorescein isothiocyanate (GOx-FITC) and catalase labeled with rhodamine B (CAT-RB) inside the large liposomes (scale bar, 5 μm). (f) Western blotting results indicating the retention of immunoreactivity of GLUT2 in the superstructures. The full gel is shown in Supplementary Figure 9. (g) CLSM image showing the reconstitution of GLUT2 labeled with rhodamine B on the membranes of the larger liposomes (scale bar, 5 μm). (h) CLSM image showing insertion of the proton channel gramicidin A labeled with lysine-5-carboxyfluorescein into the outer membrane (scale bar, 5 μm).

Journal: Nature chemical biology

Article Title: Synthetic beta cells for fusion-mediated dynamic insulin secretion

doi: 10.1038/nchembio.2511

Figure Lengend Snippet: (a) Schematic of the biochemical processes inside the AβCs. GOx, glucose oxidase; CAT, catalase; square brackets denote concentration. (b) Top, ISVs stained with uranyl acetate and imaged by TEM. The ISVs were used to mimic the insulin granules inside natural beta cells (scale bar, 100 nm). Bottom, size-distribution histogram of the insulin-containing ISVs. (c) Top, cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 5 μm). Bottom, size distribution of vesicles-in-vesicle superstructures (ISVs@OLV). (d) Magnified fractured cryo-SEM micrograph of the vesicles-in-vesicle superstructures (scale bar, 200 nm). From the fracture in d, small liposomal vesicles can be clearly seen inside the large liposome. (e) CLSM micrographs verifying the encapsulation of glucose oxidase labeled with fluorescein isothiocyanate (GOx-FITC) and catalase labeled with rhodamine B (CAT-RB) inside the large liposomes (scale bar, 5 μm). (f) Western blotting results indicating the retention of immunoreactivity of GLUT2 in the superstructures. The full gel is shown in Supplementary Figure 9. (g) CLSM image showing the reconstitution of GLUT2 labeled with rhodamine B on the membranes of the larger liposomes (scale bar, 5 μm). (h) CLSM image showing insertion of the proton channel gramicidin A labeled with lysine-5-carboxyfluorescein into the outer membrane (scale bar, 5 μm).

Article Snippet: GLUT2 expression and purification The cDNA encoding mouse GLUT2 (Origene, GenBank {"type":"entrez-nucleotide","attrs":{"text":"NM_031197","term_id":"165377236","term_text":"NM_031197"}} NM_031197 ) was amplified and cloned into the BamHI and HindIII sites of pET-28a (Novagen; full plasmid sequence in Supplementary Note ).

Techniques: Concentration Assay, Staining, Labeling, Western Blot

Effect of BG on glucose uptake in oocytes expressing human SGLT1. Oocytes expressing human SGLT1 and oocytes injected with water (SHAM, negative control) were incubated in buffer containing radiolabeled glucose only (control) or buffers containing different amounts barley BG (BG-HMW: 650,000 average molecular weight; BG-LMW: 229,000 average molecular weight). Data are means ± SEM ( n = 10–12 oocytes per treatment). *Significantly different from all other treatment groups p < 0.0001.

Journal: Frontiers in Nutrition

Article Title: Beta-Glucan From Barley Attenuates Post-prandial Glycemic Response by Inhibiting the Activities of Glucose Transporters but Not Intestinal Brush Border Enzymes and Amylolysis of Starch

doi: 10.3389/fnut.2021.628571

Figure Lengend Snippet: Effect of BG on glucose uptake in oocytes expressing human SGLT1. Oocytes expressing human SGLT1 and oocytes injected with water (SHAM, negative control) were incubated in buffer containing radiolabeled glucose only (control) or buffers containing different amounts barley BG (BG-HMW: 650,000 average molecular weight; BG-LMW: 229,000 average molecular weight). Data are means ± SEM ( n = 10–12 oocytes per treatment). *Significantly different from all other treatment groups p < 0.0001.

Article Snippet: Radiolabelled glucose ([3H] 2-deoxyglucose (25.5 Ci/mmol) and [3H] 3-O-methyl-D-glucose (25.5 Ci/mmol)] was obtained from PerkinElmer (Waltham, MA, USA). pBluescript ii KS(+) plasmids with open reading frames (ORFs) of human SGLT1 (OHu22190 NM_000343) and GLUT2 (OHu20407 NM_000340.2) were obtained from Genscript Biotech (Piscataway, NJ, USA).

Techniques: Expressing, Injection, Negative Control, Incubation, Control, Molecular Weight